Journal: bioRxiv
Article Title: Sialic acids are a barrier to the entry of non-influenza orthomyxoviruses
doi: 10.64898/2026.01.15.699645
Figure Lengend Snippet: A . Scheme of the evolution experiment. B . Representation of viral growth curves in exoNA-treated cells relative to the mock condition. The percentage of infected cells is shown. Each data point corresponds to a growth curve time point. Data are represented as a function of the mock condition instead of as a function of time to correct for differences in growth kinetics resulting from adaptations unrelated to SAs (e.g. faster VSV replication). Founder viruses are shown in blue, and the three evolved viruses in shades of red. Lines indicate a logistic growth model fit to the data by least-squares regression (goodness of fit: r 2 > 0.95 in all cases). C . Amino acid exchanges in the evolved and founder GPs. D . Clustal omega alignment of thogoto and quaranjavirus GP sequences. Only the positions that showed substitutions in our experimental evolution and their flanking regions are shown. E . Structural models of the SINUV and WBV glycoprotein trimer predicted by AlphaFold3. The exchanged positions are indicated in red. Some of the glycoprotein domains are indicated in the structures. F . Effects of exoNA treatment on the infectivity of VSV pseudotypes harboring WBV or SINUV GPs with the parental sequence or each assayed mutant. Individual data points from four independent experiments for WBV GP and from two independent experiments for SINUV GP are shown. Doses of pseudotypes were adjusted to obtain similar infectivity values among GP variants in the exoNA condition. Statistical tests comparing the effect of the exoNA treatment between GP variants are provided in the text.
Article Snippet: GP amino acid sequences were retrieved from the National Center for Biotechnology Information (NCBI) RefSeq ( www.ncbi.nlm.nih.gov/refseq ) database.
Techniques: Infection, Sequencing, Mutagenesis
